GST Bulk Kit
产品名称： GST Bulk Kit
英文名称： GST Bulk Kit
产品编号： 27-4570-01
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
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- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
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- 邮箱 : 342786402@qq.com
27-4570-01 GST Bulk Kit 27-4577-01 ANTI-GST ANTIBODY 27-4577-50 ANTI-GST ANTIBODY, 50MG 27-4583-01 PGEX-4T-3 27-4590-01 GST DETECTION MODULE 27-4810-01 PEZZ 18 EXPRESION VECTOR 28-4013-51 HIS GRAVITRAP KIT 28-4055-00 GST MULTITRAP 4B 28-4055-01 GST MULTITRAP FF 28-9523-59 GST SpinTrap 28-9523-60 GST GraviTrap 28-9523-61 GST Buffer Kit 28-9545-49 PGEX-4T-1 28-9545-50 PGEX-4T-2 28-9545-52 PGEX-4T-3 28-9545-53 PGEX-5X-1 28-9545-54 PGEX-5X-2 28-9545-55 PGEX-5X-3 28-9546-46 PGEX-2TK 28-9546-48 PGEX-6P-1 VECTOR 28-9546-50 PGEX-6P-2 VECTOR 28-9546-51 PGEX-6P-3 VECTOR 28-9546-53 PGEX-2T 25 UG WI/DI 28-9546-54 PGEX-3X 25 UG WI/DI 28-9546-56 PGEX-1LT ECOR I/BAP 11-0033-99 HIS GRAVITRAP 11-0034-00 HIS BUFFER KIT 27-4710-01 ANTI-HIS ANTIBODY 28-4009-89 HIS MULTITRAP HP 28-4009-90 HIS MULTITRAP FF GST Bulk Kit
GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins
- For rapid purification of GST-tagged proteins using gravity flow columns or using batch purification with high specificity.
- Preserves protein antigenicity and function.
- Yields up to 50 mg of pure protein.
GST Bulk Kit contains a 10 ml bulk pack of Glutathione Sepharose 4B medium, five gravity-flow columns, IPTG, and GST Buffer Kit. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the proteins. The binding capacity of Glutathione Sepharose 4B, which can be greater than 10 mg protein/ml medium, varies with the nature of the tagged protein and the exact binding conditions used.
GST Bulk Kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or a batch method. The batch method is more flexible, as purification can be performed with between 10 μl and 2 ml of Glutathione Sepharose 4B.
GST Bulk Kit also facilitates optimization studies of expression, solubility conditions, and purification parameters of GST-tagged proteins. The kit contains medium and reagents for purification of GST-tagged proteins from milliliter volumes of sample to larger volumes of sample containing as much as 50 mg of GST-tagged protein.
GST Bulk Kit — Technical Specifications
| Complete Packsize | 1 Kit |
| Includes | 10 ml bulk pack of Glutathione Sepharose 4B medium 5 x gravity-flow columns IPTG GST Buffer Kit |
| Yield | Up to 50 mg of pure protein |
| Storage Conditions | 20 - 25°C |