SNV-memTdTomato-2AcytoGFP 
(Plasmid #53124)

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• PURPOSE

  (Empty Backbone) Retrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
• DEPOSITING LAB

  Jeanette Hyer
• PUBLICATION

  Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 10.1002/dvdy.21766.
• PRICE

  $65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

• Vector backbone
  pBMN-LACZ
  (Search Vector Database)
• Backbone manufacturer
  Nolan Lab
• Vector type
  Retroviral ; expression in chicken cells
• PromoterSNV (spleen necrosis virus)
• Tags / Fusion Proteins
  • memTdTomato (C terminal on insert)
  • 2A-cyto-GFP (C terminal on insert)

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  Unknown

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ sequencing primerpBMN5
• 3′ sequencing primerEGFP-N
(Common Sequencing Primers)

RESOURCE INFORMATION

• Terms and Licenses
  • UBMTA
  • Clontech Limited Use Label License
  • Ancillary Agreement for Plasmids Containing FP Materials

DEPOSITOR COMMENTS

This plasmid is unstable during transformation. If doing a fresh transformation, confirm correct size by digest before proceeding further (~9kb).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

• For your Materials & Methods section:

  SNV-memTdTomato-2AcytoGFP was a gift from Jeanette Hyer (Addgene plasmid # 53124)

• For your References section:

  Murine retroviruses re-engineered for lineage tracing and expression of toxic genes in the developing chick embryo. Venters SJ, Dias da Silva MR, Hyer J. Dev Dyn. 2008 Nov;237(11):3260-9. doi: 10.1002/dvdy.21766.10.1002/dvdy.21766 PubMed 18942139