信号通路Wnt11抗体-软件手册-资讯-生物在线

信号通路Wnt11抗体

作者:上海雅吉生物科技有限公司 2020-11-09T00:00 (访问量:943)

 

研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 36kDa
细胞定位 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Wnt11:151-250/354 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene ”wingless” and to its vertebrate ortholog, Int-1, a mouse proto-oncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins which have features typical of secreted growth factors. Wnt-11 is expressed in the tips of ureteric buds and in the perichondrium, a stem cell-like layer that surrounds the future bones and directs their growth and regeneration. Wnt-11 activity is required for cells to undergo correct convergent extension movements during gastrulation. Human Wnt-11 is also expressed in the lung mesenchyme, the urorectal septum, the urogenital folds, the labioscrotal swellings, and the cortex of the adrenal gland. Unlike other Wnt family members, Wnt-11 is not expressed in the neuroepithelium of the central nervous system. Wnt-11, along with Wnt-8c, is ex-pressed in the posterior region of the chick embryo in the caudal paraxial mesoderm that underlies the prospective caudal neural plate. The gene which encodes Wnt-11 maps to human chromosome 11q13.5.

Function:
Wnt11 is a non-canonical Wnt which is expressed in androgen-independent prostate cancer cell lines and prostate tumours. It is involved in development, including cardiac and gut development.

Subcellular Location:
Secreted; extracellular space; extracellular matrix.

Tissue Specificity:
Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas.

Post-translational modifications:
Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-80. Palmitoylation is necessary for proper trafficking to cell surface (By similarity).

Similarity:
Belongs to the Wnt family.

SWISS:
O96014

Gene ID:
7481

Database links:

Entrez Gene: 7481 Human

Entrez Gene: 22411 Mouse

Entrez Gene: 140584 Rat

Omim: 603699 Human

SwissProt: O96014 Human

SwissProt: P48615 Mouse

 



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample:
Embryo (Mouse) Lysate at 40 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Wnt11 (bs-8568R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- Wnt11 (bs-8568R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 63 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Wnt11 (bs-8568R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Wnt11) Polyclonal Antibody, Unconjugated (bs-8568R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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