溶质载体家族30成员8(SLC30A8)重组蛋白说明书-分析方法-资讯-生物在线

溶质载体家族30成员8(SLC30A8)重组蛋白说明书

作者:上海沪震实业有限公司 2023-04-16T00:00 (访问量:318)

 溶质载体家族30成员8(SLC30A8)重组蛋白说明书

Recombinant Solute Carrier Family 30 Member 8 (SLC30A8) 

[ PROPERTIES ]

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Source: Prokaryotic expression

Host: E.coli

Residues: Thr263~Asp369

Tags: Two N-terminal Tags, His-tag and SUMO-tag

Subcellular Location: Secreted

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 50µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 25.4kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in ddH2Oto a concentration of 0.1-0.25 mg/mL. Do not vortex

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

 

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

 

 

 

[ SEQUENCE ]

2022052504062414222

[ IDENTIFICATION ]

2022052504063074940

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用

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