食道癌抑制生长蛋白抗体-分析方法-资讯-生物在线

食道癌抑制生长蛋白抗体

作者:上海雅吉生物科技有限公司 2020-11-03T00:00 (访问量:910)

  

中文名称 食道癌抑制生长蛋白抗体
别    名 1500011J06Rik; arginine/serine-rich coiled-coil 2; FLJ11021; RSRC2_HUMAN.  
研究领域 肿瘤  细胞生物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50 kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RSRC2:351-434/434 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 RSRC2 (arginine/serine-rich coiled-coil 2) possibly plays a role in cell proliferation and may be a novel tumor suppressor of esophageal cancer cell growth.

Similarity:
Belongs to the RSRC2 family.

SWISS:
Q7L4I2

Gene ID:
65117

Database links:

Entrez Gene: 65117 Human

Entrez Gene: 208606 Mouse

Entrez Gene: 360807 Rat

SwissProt: Q7L4I2 Human

SwissProt: A2RTL5 Mouse

SwissProt: Q5PQR4 Rat

Unigene: 432996 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-RSRC2 (bs-6097R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- RSRC2 (bs-6097R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSRC2) Polyclonal Antibody, Unconjugated (bs-6097R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSRC2) Polyclonal Antibody, Unconjugated (bs-6097R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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