YAP1抗体Applications
| In-house applications |
ELISA, IHC, FC, IF, IP, WB Application not tested? Please see our Guarantee. |
| Published applications |
FC, IF, IHC, WB |
Publications |
11 citations –view here |
| Species specificity |
Human, Mouse, Zebrafish; other species not tested. |
| Published Species |
Human, mouse, zebrafish |
|
Images
Prev12Next
 Immunohistochemical of paraffin-embedded human ovary tumor using 13584-1-AP(YAP1 antibody) at dilution of 1:100 (under 40x lens) FC result of YAP antibody (13584-1-AP) with Min6 and NIH3T3 cell by Sarvetnick Lab, UNMC HeLa cells were subjected to SDS PAGE followed by western blot with 13584-1-AP( YAP1 Antibody) at dilution of 1:1000 IP Result of anti-YAP1 (IP:13584-1-AP, 3ug; Detection:13584-1-AP 1:700) with NIH/3T3 cells lysate 1200ug. Immunofluorescent analysis of HepG2 cells using 13584-1-AP( YAP1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
|
| Positive WB detected in |
HeLa cells, MCF-7 cells |
| Positive IP detected in |
NIH/3T3 cells |
| Positive IHC detected in |
Human ovary tumor tissue |
| Positive IF detected in |
HepG2 cells |
| Positive FC detected in |
NIH3T3 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:50-1:200
IF : 1:20-1:200 |
YAP1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information YAP1抗体
| Immunogen |
YAP1 fusion protein ag4510 |
Full name |
Yes-associated protein 1, 65kDa |
Calculated molecular
weight |
504aa,54 kDa |
Observed molecular
weight |
65-70 kDa |
GenBank accession
number |
BC038235 |
Gene ID (NCBI) |
10413 |
| Gene symbol |
YAP1 |
Synonyms |
65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog |
Background
|
Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).This antibody is a rabbit polyclonal antibody. YAP1抗体
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