TRIM21抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
TRIM21抗体

TRIM21抗体

商家询价

产品名称： TRIM21抗体

英文名称： TRIM21Antibody

产品编号： XY 12108-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 TRIM21抗体Applications

In-house applications ELISA, WB, IHC, IP, IF     Application not tested? Please see our Guarantee.
Published applications WB Publications 1 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 12108-1-AP;A549 cellA549 cells were subjected to SDS PAGE followed by western blot with 12108-1-AP(TRIM21 antibody) at dilution of 1:500
  • 12108-1-AP;Lung cancerImmunohistochemical of paraffin-embedded human lung cancer using 12108-1-AP(TRIM21 antibody) at dilution of 1:50 (under 10x lens)
  • 12108-1-AP;A549 cellsIP Result of anti-TRIM21 (IP:12108-1-AP, 4ug; Detection:12108-1-AP 1:500) with A549 cells lysate 920ug.
  • 12108-1-AP;A549 cellsImmunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 12108-1-AP( TRIM21 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in A549 cells, HeLa cells, HL-60 cells, HT-1080 cells, mouse pancreas tissue
Positive IP detected in A549 cells
Positive IHC detected in Human lung cancer tissue
Positive IF detected in A549 cells
Recommended dilution WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500
 TRIM21抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information TRIM21抗体
Immunogen TRIM21 fusion protein ag2753 Full name Tripartite motif-containing 21
Calculated molecular
weight		 475aa,54 kDa Observed molecular 
weight		 52 kDa
GenBank accession 
number		 BC010861 Gene ID (NCBI) 6737
Gene symbol TRIM21 Synonyms 52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21
Background

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21抗体

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