TP53抗体Applications
| In-house applications |
ELISA, IP, WB, IF, IHC Application not tested? Please see our Guarantee. |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
|
Images
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 WB result of p53 antibody (21891-1-AP, 1:1000) with sh-control and sh-p53 transfected A431 cells. A431 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP(TP53 antibody) at dilution of 1:1000 IP Result of anti-TP53 (IP:21891-1-AP, 3ug; Detection:21891-1-AP 1:1000) with A431 cells lysate 1650ug. Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 21891-1-AP( TP53 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). Immunofluorescent analysis of HepG2 cells using 21891-1-AP( TP53 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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| Positive WB detected in |
A431 cells, HEK-293 cells, NIH/3T3 cells |
| Positive IP detected in |
A431 cells |
| Positive IHC detected in |
Human endometrial cancer tissue, human ovary tumor tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:50-1:500
IF : 1:20-1:200 |
TP53抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information TP53抗体
| Immunogen |
TP53 fusion protein ag16596 |
Full name |
Tumor protein p53 |
Calculated molecular
weight |
393aa,44 kDa |
Observed molecular
weight |
53 kDa |
GenBank accession
number |
BC003596 |
Gene ID (NCBI) |
7157 |
| Gene symbol |
TP53 |
Synonyms |
Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
Background
|
TP53, also named as P53 and NY-CO-13, belongs to the p53 family that functions as a central mediator for organizing cellresponses to various stress and anticancer drugswith apoptosis,G1- phase arrest, and DNA repair.[PMID: 20346922]. In addition, TP53 appears to induce apoptosis through nontranscriptional cytoplasmic processes. In unstressed cells, TP53 is kept inactive essentially through the actions of the ubiquitin ligase MDM2, which inhibits TP53 transcriptional activity and ubiquitinates TP53 to promote its degradation[PMID: 22330136]. TP53 acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53抗体
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