英文名称：SCN10A/Nav1.8
中文名称：钠通道蛋白10α抗体
别    名：mPN3; Peripheral nerve sodium channel 3; Pn3 (gene name)v PN3; Scn10a; Sensory neuron sodium channel; Sns (gene name); SNS; sodium channel protein type 10 subunit alpha; Sodium channel protein type X alpha subunit; Voltage-gated sodium channel alpha subunit Nav1.8; NAV1.8.
 
规格价格：0.1ml/800元   0.2ml/1280元 
说 明 书：0.1ml  0.2ml  
研究领域：神经生物学  通道蛋白  细胞膜受体  
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, 
产品应用：  WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.
 
分 子 量：215kDa
细胞定位：细胞膜、细胞外基质
性    状：Lyophilized or Liquid
浓    度：1mg/1ml
免 疫 原：KLH conjugated synthetic peptide derived from human SCN10A/N亚    型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
产品介绍：background:

Nav1.8 protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Nav1.8 forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Nav1.8 plays a role in neuropathic pain mechanisms.



Function:

This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Its electrophysiological properties vary depending on the type of the associated beta subunits (in vitro). Plays a role in neuropathic pain mechanisms.



Subunit:

The voltage-resistant sodium channel consists of an ion conducting pore forming alpha-subunit regulated by one or more associated auxiliary subunits SCN1B, SCN2B and SCN3B. Found in a number of complexes with PRX, DYNLT1 and PDZD2. Interacts with proteins such as FSTL1, PRX, DYNLT1, PDZD2, S100A10 and many others. Interacts with NEDD4 and NEDD4L.



Subcellular Location:

Membrane; Multi-pass membrane protein. Note=It can be translocated to the extracellular membrane through association with S100A10.



Tissue Specificity:

Expressed in the dorsal root ganglia and sciatic nerve.



Post-translational modifications:

Ubiquitinated by NEDD4L; which promotes its endocytosis.



Similarity:

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.

Contains 1 IQ domain.



Database links:
Entrez Gene: 6336 Human
Entrez Gene: 20264 Mouse
Entrez Gene: 29571 Rat
Omim: 604427 Human
SwissProt: Q9Y5Y9 Human
SwissProt: P70276 Mouse
SwissProt: Q6QIY3 Mouse
SwissProt: Q62968 Rat
Unigene: 250443 Human
Unigene: 10246 Rat




Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品图片
 

Antigen: bs-6685P, 0.2ug/100ul 

Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; 

Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; 

TMB(C-0024) staining; 

Read the data in MicroplateReader by 450nm. 

Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded; 

Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 

Incubation: Anti-SCN10A Polyclonal Antibody, Unconjugated(bs-6685R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; 

Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 

Incubation: Anti-SCN10A Polyclonal Antibody, Unconjugated(bs-6685R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.