NF-κB抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
NF-κB抗体

NF-κB抗体

商家询价

产品名称： NF-κB抗体

英文名称： NF-κBAntibody

产品编号： XY14220-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 NF-ΚB抗体Applications

In-house applications ELISA, WB, IF, IP     Application not tested? Please see our Guarantee.
Published applications IF, IP, WB Publications 5 citations –view here
Species specificity Human, Rat; other species not tested.
Published Species Human, rat
Images
Prev1NextView more validation data
  • 14220-1-AP;Jurkat cellsJurkat cells were subjected to SDS PAGE followed by western blot with 14220-1-AP(NFKB1 antibody) at dilution of 1:1000
  • 14220-1-AP;Jurkat cellsIP Result of anti-NFKB1 (IP:14220-1-AP, 3ug; Detection:14220-1-AP 1:500) with Jurkat cells lysate 1800ug.
  • 14220-1-AP;HeLa cellsImmunofluorescent analysis of HeLa cells using 14220-1-AP( NFKB1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
Positive WB detected in Jurkat cells, HeLa cells, K-562 cells, Raji cells
Positive IP detected in Jurkat cells
Positive IF detected in HeLa cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
IF : 1:20-1:200
 NF-ΚB抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen NF-κB fusion protein ag5458 Full name Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Calculated molecular
weight		 105 kDa Observed molecular 
weight		 50 kDa and 105 kDa
GenBank accession 
number		 BC051765 Gene ID (NCBI) 4790
Gene symbol NFKB1 Synonyms DKFZp686C01211, DNA binding factor KBF1, EBP 1, KBF1, NF kappa B, NFKB p105, NFKB p50, NFKB1, NFKB1,p105, NFKB1,p105,p50, NF-κB, p105, p50
Background NF-ΚB抗体

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1. NF-ΚB抗体

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