HIF1A抗体
Applications
| Tested Applications: |
ELISA, IHC, IF, FC, WB |
| Cited applications |
Co-IP, IHC, WB |
| Specificity Reaction: |
Human,Mouse,Rat; Other species are not tested.Please decide the specificity by homology |
| Cited species |
Human, mouse, rat |
| Positive WB detected in |
Cobalt Chloride treated HeLa cells, HeLa cells, mouse small intestine tissue |
| Positive IHC detected in |
Human thyroid cancer tissue, human heart tissue, human kidney tissue |
| Positive IF detected in |
Hela cells |
| Positive FC detected in |
HeLa cells |
| Recommended dilution |
WB : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100
FC : N/A |
HIF1A抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP( HIF1a Antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using HIF1A antibody 20960-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemical of paraffin-embedded human thyroid cancer using 20960-1-AP(HIF1A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human thyroid cancer using 20960-1-AP(HIF1A antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug HIF1a antibody (20960-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Product Information HIF1A抗体
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information
| Immunogen: |
HIF1a fusion protein ag15198 |
Full Name: |
Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
Caculated Molecular
Weight: |
826aa,93 kDa |
Observed Molecular
Weight: |
120 kDa |
| GenbankNo |
BC012527 |
GeneID: |
3091 |
| Symbol |
HIF1A |
Synonyms |
ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, Member of PAS protein 1, MOP1, PASD8 |
Background
|
HIF1, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1alpha (HIFA), and an oxygen-insensitive subunit, HIF1beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Although the mRNA is constitutively and ubiquitously expressed and transcribed, the HIF1A protein is rapidly degraded in the presence of oxygen. The half-life of HIF1A under ambient oxygeconditions is less than 5 min. Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway. HIF1A抗体
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