Is the antibody always to blame?

  • 来源:生物谷
  • 时间: 2020/10/21
  • 浏览人数: 554

     Is the antibody always to blame? 

    In the example blot to the right, the same antibody was used (Anti-BAK1, AS12 1858) but the proteins were extracted with two different buffers. 
    Minor adjustments in the buffer components resulted in the improved detection of the target protein - from no band detected at all as seen in the left panel to a band of correct MW visible in the right panel. 

    If you are interested to learn more how to optimize your western blot, you are welcome to contact us or request a copy of the Agrisera Western Blot Guide.
     


     

      Western blot using anti-BAK1 antibodies