免疫荧光

  • 来源:生物谷
  • 时间: 2006/6/8 21:03:00
  • 浏览人数: 9247

    ·         Staining for total receptor(Von Zastrow lab)
    This protocol will visualize both internalized receptors that were once on the plasma membrane and receptors in the biosynthetic pathway. This protocol was specifically written for visualizing agonist-induced internalization of receptors.

      

    ·         Antibody Cleanup(YGAC)
    In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use.