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DIG Southern Blot Solutions

来源:生物谷  2010/7/2   访问量:2637
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0.25M HCl (1L)

  • 20.66mL HCl
  • 979.34mL dH2O
  • *add acid to water*

1M ammonium acetate, 0.02M NaOH (1L)

  • 77.08g ammonium acetate
  • 0.8g NaOH
  • Fill with dH2O

0.5X SSC, 0.1% SDS (1L)

  • 25mL 20X SSC
  • 10mL 10% SDS
  • 965mL dH2O

10X Blocking Buffer Þ 1X (30mL)

  • 3mL Blocking Buffer stock
  • 27mL Maleic Acid Buffer

Wash Buffer 3 (30mL)

  • 30mL Maleic Acid Buffer
  • 90µ l Tween 20 (cut off pipet tip)

Detection Buffer (1L)

  • 100mL 1M Tris pH 9.5
  • 20mL 5M NaCl
  • 880mL dH2O
  • *Autoclave*

1.5M NaCl, 0.5M NaOH (1L)

  • 87.66g NaCl
  • 20g NaOH
  • Fill with dH2O

2X SSC, 0.1% SDS (1L)

  • 100mL 20X SSC
  • 10mL 10% SDS
  • 890mL dH2O

Maleic Acid Buffer (1L)

  • 11.61g Maleic Acid
  • 8.766g NaCl
  • Fill with dH2O
  • *check pH 7.5 with NaOH pellets (~7g)
  • *Autoclave*

Diluting Ab 1:10,000 (30mL)

  • 3mL Blocking Buffer stock
  • 27mL Maleic Acid Buffer
  • 3µ l Ab

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